Firstly, try another PS. If that doesnt work, it could be hardware related.
Similar thing happened to me, once i changed it, everything was perfect again. That PS was almost 5 years old anyway.
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Vista Ultimate x64, Core 2 Quad 2.4GHz Q6600, 4GB DDR2 Crucial, 500GB Hitachi SATAII, nVidia GeForce 8800 GTX, Asus P5N32-E SLI PLUS, 650W Sweex PSU
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